Mitshison Lab, Department of Systems Biology, Harvard Medical School
The key variable in MT spindown experiments is ATP. Under high ATP conditions,conventional MAPs are selectively co-sedimented with microtubules. In theabsence of ATP (or in presence of AMPPNP which induces rigor binding of motorsto MTs), both motors and MAPs will bind to MTs and pellet with themicrotubules.……
http://mitchison.med.harvard.edu/protocols/mt2.html
