Ron Vale Mt Polymerization Buffer
- 点击: 作者:51protocol收集 来源: 日期:2007-04-29 本站论坛
Koshland Lab,Carnegie Institute
http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/2xmicropoly.html
Ron Vale Buffer - 2X Microtubule Polymerization Mix
- 1 ml
200 µls 5X BRB 80
200 µls DMSO
8 µls 1M MgCl2
20 µls 0.1 M GTP (KOH)
572 µls dH2O
- Flash Freeze into Small Aliquots (50 µls)
- Store in the -70oC
- Use only once then discard
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