Koshland Lab,Carnegie Institute http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/2xmicropoly.html Ron Vale Buffer - 2X Microtubule Polymerization Mix 1 ml 200 ls 5X BRB 80 200 ls DMSO 8 ls 1M MgCl 2 20 ls 0.1 M GTP (KOH) 572 ls dH 2 O Flash
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  • Ron Vale Mt Polymerization Buffer

  • 点击:    作者:51protocol收集   来源: 日期:2007-04-29    本站论坛
Koshland Lab,Carnegie Institute http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/2xmicropoly.html
Ron Vale Buffer - 2X Microtubule Polymerization Mix
  1. 1 ml
    200 µls 5X BRB 80
    200 µls DMSO
    8 µls 1M MgCl2
    20 µls 0.1 M GTP (KOH)
    572 µls dH2O
  2. Flash Freeze into Small Aliquots (50 µls)
  3. Store in the -70oC
  4. Use only once then discard

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