细胞组分和细胞器——细胞骨架

• Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab)
    
• Recycling Tubulin (Mitchison Lab)
    
• Labeling Tubulin and Quantifying Labeling Stoichiometry (Mitchison Lab)
    
• Tubulin Polymerization with GTP/GMPCPP/Taxol (Mitchison Lab)
    
• Preparation of Segmented and Polarity Marked Microtubules (Mitchison Lab)
   
• Microtubule Spindowns for Visual Analysis (Mitchison Lab)
    
• Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC (Mitchison Lab)
   
• Negative Stain Electron Microscopy of Microtubules (Mitchison Lab)
    
• Microtubule Pelleting (Mitchison Lab)
    
• Actin - Chromatography Purification (The Cell Biology and Cytoskeleton Group, HMS)
     
• Actin - Labeling with Pyrene Iodoacetamide (The Cell Biology and Cytoskeleton Group, HMS)
     
• Actin Binding Assay with Rhodamine-Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
     
• Actin Extraction from Acetone Powder (The Cell Biology and Cytoskeleton Group, HMS)
     
• Actin Preparation - Acetone Powder Method (The Cell Biology and Cytoskeleton Group, HMS)
• Plasma Gelsolin Preparation (The Cell Biology and Cytoskeleton Group, HMS)
• Some Buffers for Actin Work (The Cell Biology and Cytoskeleton Group, HMS)
     
• Fixing and Immunostaining Cells (The Cell Biology and Cytoskeleton Group, HMS)
    
• Fixing and Staining Cells for Actin with Fluorescently-Labeled Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
• "Cells on Gels" (The Cell Biology and Cytoskeleton Group, HMS)
  this protocol describes method for culture cell on a thin polyacrylamide-based, collagen-coated flexible substrate. By maintaining a constant total concentration of acrylamide while usr/localying the concentration of bis-acrylamide, it's able to obtain a series of chemically identical substrates with a wide range of flexibility. By using imaging techniques,  cells' response to differences in substrate flexibility can be detected. 
    
• Immunofluorescent Localization of Tubulin (William H. Heidcamp)
  For cultured cell
    
• Isolation of Microtubules (Bovine Brain) (William H. Heidcamp)
    
• Viscosity & Polymerization of Microtubules (William H. Heidcamp)
     
• TEM Visualization of Microtubules (William H. Heidcamp) 
    
• SDS Gel Electrophoresis of Tubulin\MAPs(William H. Heidcamp)
    
• Isolation of Actin and Myosin Filaments (William H. Heidcamp)
     
• Yeast Actin Capture Assay (D. Amberg)