· Comparative Genomic Hybridization (CGH) CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. This protocol gives detailed procedures on CGH as follows:
Metaphase chromosome preparation
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Hybridization
DNA detection
Image capture
Image analysis
DAPI chromosome identification
· Comparative Genomic Hybridization (CGH)
Detailed protocol for CGH, including slide and probe preparation, detection and staining
- Direct CGH (Waldman Lab)
- Indirect CGH (Waldman Lab)
- Nick Translation of DNA for CGH (Waldman Lab)
