胚胎干细胞培养

• Media and Solution required for ES Cell Culture (Bowtell Lab)
     
• Routine Culturing of ES Cells (Bowtell Lab)
    
• Routine Splitting and freezing of cells (PMCI)
     
• ES Cell Freezing and Thawing (Nagy Lab)
    
• ES Cell Culture and Manipulation (The Cancer Center at Washington University)
• Cell Culture from Whole Mice Embryo  (PMCI)
  Obtain ES cell from mice embryo for culture
    
• ES Cell Culture and Manipulation (Chisholm Lab)
  Detailed protocol for ES cell manipulation, including Media, plating ES cell, Splitting ES cells, freezing ES cells, transformation of ES cells, expansion of ES cell clones and more...
• Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos (Nagy Lab)
  To produce completely ES cell-derived embryos a clump of ES cells is sandwiched between two tetraploid embryos. For ES cell aggregation chimeras with diploid embryos, one embryo is aggregated with a clump of ES cells. Even in this case, the ES cell internalization is efficient enough to achieve a high level of ES cell contribution.  
     
• Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos (Nagy Lab) 
     
• ES / MEF cell culture and electroporation of targeting construct (Gary Brown)
    
• Preparation of Murine Embryonic Fibroblasts (Gary Brown)
    
• Isolation of Primary Mouse Embryo Fibroblasts (PMCI)
      
• Isolation of Primary Mouse Embryo Fibroblasts (Bowtell Lab)
    
• Electroporation of ES Cells and Isolation of H/R Clones  (Bowtell Lab)
      
• Electroporation of ES Cell (Nagy Lab)
    
• Karyotyping ES Cells (Bowtell Lab)
     
• Vasectomy of Male Mice (Bowtell Lab)
    
• Media for Embryo Culture and Manipulation (Bowtell Lab)
    
• Aggregation of ES Cells and Eight Cell Stage embryos (Bowtell Lab)
    
• Blastocyst Transfer (Bowtell Lab)
    
• ES / MEF Cell Culture and Electroporation of Targeting Construct (Gary Brown)
    
• In Vitro Differentiation of ES Cells into: (Nagy Lab)
  • Cardiac Muscle
  • Neuronal Lineages
  • Cystic Embryoid Bodies
     
• Mitomycin C Treatment of PMEFs (PMCI)
    
• Gamma Irradiation of PMEF'S (PMCI)
    
• PMEF Feeder Layer Concentration (PMCI)
    
• FAQs for Embyonic Stem Cell Culture (LTI)
  very useful information about many aspects of ES cell culture
      
• Normaski Optics (Bowtell Lab)
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