hoechst33258染色,他们认为细胞发生凋亡时,染色质会固缩。 所以Hoechst染色时,细胞核会呈致密浓染,或呈碎块状致密浓染。
正常细胞核

刺激后有致密浓染的凋亡细胞
AO-EB染色法:
The image below shows human lymphoma cells treated with the chemotherapy agent camptothecin. The cells that are undergoing apoptosis appear yellow and show the characteristic membrane blebbing (bubble formation)seen in cells dying via apoptosis.
Promega公司的DeadEnd
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