Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling)
laboratory of experimental pathology,NIH
The ISEL technique identifies morphologically apparent karyorrhetic cells and also labels cells in which the DNA fragmentation has not yet progressed to frank nuclear fragmentation. By using biotinylated nucleotides and a DNA polymerase, the DNA fragments serve as templates in situ to synthesize new DNA strands, which are visualized by light microscopy after histochemical processing.
http://dir.niehs.nih.gov/dirlep/ish/isel1.html
