Apoptosis In Situ Detection Using Terminal deoxynucleotidyl Transferase (TdT)
Lab of experimental pathology,NIH
Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during apoptosis. Incorporation of biotinylated dUTP allows detection by immunohistochemical procedures. The labeled apoptotic cells may be visualized by light microscopy
http://dir.niehs.nih.gov/dirlep/ish/tunel1.html
