Hybridization Histochemistry- 点击: 作者:51protocol收集 来源: 日期:2007-03-28 本站论坛
J. Histochem. Cytochem. 39: 1377-1384.
Morey, A.L. 1995. Non-isotopic in situ hybridization at the ultrastructural level.J. Pathol. 176:113-121.
Rogers, A.W. 1979. Techniques of Autoradiography. Elsevier, New York.
Rosen, B. and Beddington, R.S. 1993. Whole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions.Trends Genet. 9:162-167.
Swiger, R.R. and Tucker, J.D. 1996. Fluorescence in situ hybridization: a brief review.Environment. Mol. Mutagen. 27:245-254.
Valentino, K.L., Eberwine, J.H. and Barchas, J.D. (eds.). 1987. In Situ Hybridization. Applications to Neurobiology. Oxford University Press, New York.
Westlake, T.M., Howlett, A.C., Bonner, T.I., Matsuda, L.A. and Herkenham, M. 1994. Cannabinoid receptor binding and messenger RNA expression in human brain: an in vitro receptor autoradiography and in situ hybridization histochemistry study of normal aged and Alzheimer's brains.Neuroscience 63:637-652.
Wilkinson, D.G. (ed). 1992. In Situ Hybridization. A Practical Approach. Oxford University Press, New York.
Young, W.S. III. 1992. In situ hybridization with oligodeoxyribonucleotide probes. In In Situ Hybridization. A Practical Approach (Wilkinson, D.G., ed) pp. 33-44. Oxford University Press, New York
Young, W.S. III., Mezey, É . and Siegel, R.E. 1986. Vasopressin and oxytocin mRNAs in adrenalectomized and Brattleboro rats: analysis by quantitative in situ hybridization histochemistry.Mol. Brain Res. 1:231-241.
Young, W.S. III., Reynolds, K., Shepard, E.A., Gainer, H. and Castel, M. 1990. Cell-specific expression of the rat oxytocin gene in transgenic mice. J. Neuroendocrinol.2:917-925.
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Photomicrograph shows oxytocin and vasopressin neurons within the human supraoptic nucleus labeled with 35S-labeled and digoxigenin-labeled probes, respectively. Neurons containing the digoxigenin-labeled probe show a dark stain from development of the alkaline phosphatase on the anti-digoxigenin antibodies. The deposition of the radiolabeled probe is indicated by the green-colored silver grains. Click on the image to see an enlarged version. |
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