Non-Radioactive In Situ Hybridization to Sections非放射性原位杂交【Harvard Unive

PBS

5'

PFA

15'

4% (50ml of 20% PFA in 250ml PBS)

PBS

5'

PK

5'

1 礸/ml (25l of 10mg/ml PK in 250ml PBS)

Glycine

5'

~2 mg/ml (~0.5g in 250ml PBS)

PBS

5'

PFA

15'

4% in PBS

Acetic Anhydride

10'

0.25% (625l in 250ml of 0.1M TEA)

PBS

5'

Hyb Buffer

HybBff

Probe

1 Cleaned Tx Rxn in 15ml of HybBff

Prewash