• The TRC shRNA Design Methods and Rules [TRC,broad institute,MIT]

• 点击：    作者：51protocol收集   来源： 日期：2007-03-28    本站论坛

Rules

• avoid SNPs
• THREE PRIME CLAMP
• FIVE PRIME CLAMP
• GC content
• avoid-four-in-a-row
• avoid seven (G|C) in a row
• internal loop penalty
• internal AT skew
• internal AT flanking skew
• penalize AA start
• penalize non-GATC bases