Gregory Hannon,Cold spring harbor lab
Here is our T7 siRNA protocol. The main idea is to design primers that begin with GG so thattranscription by T7 polymerase is efficient.
http://katahdin.cshl.org:9331/homepage/portal/html/protocols/T7si_Protocol_v1.11.pdf
上一篇:The pSico and pSicoR Design and Cloning 设计与克隆【MIT】 下一篇:The TRC shRNA Design Methods and Rules [TRC,broad institute,MIT]
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