Hello,
I'm trying to knockdown the amount of a nuclear RNA. Does the Dicer-mediated degradation of the targeted RNA occur in the nucleus or in the cytoplasm??
If it occurs in the cytoplasm, does polyadenylated nuclear RNA get exported to the cytoplasm before it is localized to the nucleas??
Is knocking down a nuclear RNA feasible?
Thanks a lot,
Nick
-Nick-
There is a very recent paper reporting RNAi works in the nucleus. The abstract is here
-pcrman-
my experience is that in the nucleus, antisense works a lot better than RNAi.
-expresson_help-
hi
a recent paper may help you :
they worked on mouse embryo
and you may have a look at this topic
hope that helps
Fred
-fred_33-
this paper may help you
-Elea-
a recent paper may help you :
they worked on mouse embryo
-cristian-
