WARNING! The procedure for creating input files for Genomic Primers has been changed! Please refer to the 'how to obtain GenBank files with genomic sequence' link below.
Welcome to the PCR Suite: a collection of programs that interact with the Whitehead's . The original Primer3 program designs primersets for one sequence and it allows you to change almost every parameter. The PCR Suite has more stringent settings (to allow more working primersets) and a smaller range of parameters (because you won't change most of them anyway).
The suite consists of four programs:
is for creating multiple overlapping PCR products in one sequence.
designs primers around exons in genomic sequence. All you need is a GenBank file containing your gene.
designs primers around every SNP in a GenBank file.
designs primers around open reading frames. Simply upload a GenBank file containing your genes.
if you want to know how to obtain GenBank files with
genomic sequence.
if you want to know how to obtain GenBank files with
cDNA sequences.
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