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  • Avoiding DNA Contamination in RT-PCR

  • 点击:    作者:   来源: 日期:2007-03-07    本站论坛

In addition to DNA-free, Ambion offers many quality products to facilitate successful RT-PCR experiments. These include RNase-free pipette tips and PCR tubes, RNase free DNase I, ToTALLY RNA, RNAqueous, and Poly(A)Pure RNA Isolation Kits, RETROscript First Strand cDNA Synthesis Kit, and SuperTaq?thermostable DNA polymerase. All of Ambion's products designed for use with RNA undergo rigorous quality testing and are certified RNase-free.

The Polymerase Chain Reaction (PCR) is covered by patents owned by Hoffman-LaRoche. Use of the PCR process requires a license. A license for research may be obtained by purchase and use of authorized reagents and DNA thermal cyclers.

SuperTaq™ is made by Enzyme Technologies Limited and sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc. and the Perkin-Elmer Corporation. Ambion is a distributor of Enzyme Technologies Limited.

Purchase of SuperTaq is accompanied by a limited license for its use in the Polymerase Chain Reaction (PCR) and RT-PCR process for research in conjunction with a thermal cycler, the use of which in the automated performance of the PCR and RT-PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer, or as purchased, i.e., an authorized thermal cyler.

Super Taq is not available for sale directly from Ambion in the United Kingdom, France, BeNeLux, Denmark, Sweden, Italy, Austria, Switzerland, Singapore, and Taiwan. Contact Enzyme Technologies LTD, Unit 4, 61 Ditton Walk, Cambridge CB5 8QD, U.K. (phone 44-1223-412-583) for more information.


 



 



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