Protocol for mRNA amplification，非常详细的RT-PCR实验过程[Howard Hughes Medical
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2ul	0.1M DTT
1ul	RNaseIN (Promega Cat# N2111)
2ul	10mM dNTP (Pharmacia Cat# 27-2035-02)
2ul	Superscript II (Gibco BRL Cat# 18064-071)

42C for 90min in thermal cycler.

From here, follow the procedure of first round amplification for

Second strand synthesis,

DS cDNA cleanup,

In Vitro TRanscription,

and aRNA purification using RNEasy.

Note: when labeling amplified RNA - USE RANDOM HEXAMERS.