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Protocol for mRNA amplification，非常详细的RT-PCR实验过程[Howard Hughes Medical

点击：   作者：51protocol收集   来源：  时间： 2007-03-19 　本站论坛

Reagent

0.5-1ug

aRNA from 1st round

9ul

DEPC H2O

1ul

(2ug/ul) random hexamer (i.e. dN6)

70C for 3min, cool to room temperature.

Add to PCR Tube (separately or in a Master Mix)

Ammt

Reagent

4ul

5X First strand buffer

1ul

(1ug/ul) oligo dT-T7 primer


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