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Protocol for mRNA amplification，非常详细的RT-PCR实验过程[Howard Hughes Medical

点击：   作者：51protocol收集   来源：  时间： 2007-03-19 　本站论坛

Reagent

2 ul

of each 75mM NTP (A, G, C and UTP)

2 ul

Reaction buffer

2 ul

Enzyme mix (RNase inhibitor and T7 phage polymerase)

8 ul

ds cDNA in H20

Add 20uL to each tube.

Incubate at 37C for 5-6hr.

aRNA purification using Qiagen RNeasy COLUMNS

Make up RLT Buffer Master Mix with b-ME and H2O.

Ammt

Reagent

3.5 ul


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