| Large-scale yeast transformation大规模转化酵母【Yale University】 | | 点击： 作者：51protocol收集 来源： 时间： 2007-03-25 本站论坛 |
|  | Make a little hole in the sidewall at each end of the Beckman boxes, to allow the air to escape.
Microplate carriers for centrifuge that allow you to spin deepwell boxes: For this, the microplate carriers must lack a crossbrace. We have a Sorvall T6000D, with H-1000B rotor. With this we use braceless microplate carriers (#11093) and also two earlier carriers that I removed the crossbraces from (#11065). The only apparent problem with removing the cross braces is that now they're slightly imbalanced (and they make me nervous).
Nunc Omnitrays: Rectangular petri dish, handy for using 12 channel pipettor to plate. (Fisher #12-565-296; $34/60). These can be washed with water, soaked in 3% hydrogen peroxide, and reused.
8 or 12 channel manifold pipettor.
Large orifice tips for mixing PEG.
Reservoirs for holding liquid for multichannel pipettors.
Microplate attachment for vortexer.
上一篇：Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌 下一篇：Electro-Transformation of Yeast电转酵母【Upstate Medical University】
共2页: 上一页 [1] 2 下一页 | | |
|
