Transformation of E. coli by Electroporation电穿孔转化E.coli【University of Texa
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  • Transformation of E. coli by Electroporation电穿孔转化E.coli【University of Texa

  • 点击:    作者:51protocol收集   来源: 日期:2007-03-25    本站论坛
  • For additional samples, move chamber select knob and repeat electroporation as above.
  • Turn off power buttons on both the power booster and pulse contol and disconnect cable on chamber safe. Remove suspended cells from chambers with a pipet and place in 1 mL rich media (SOC) to recover for 1 hr. at 37 °C in a shaker.
  • Plate 150 µL on appropriate plates (with antibiotic that corresponds to the resistance offered by your plasmid). Let grow overnight in 37 °C incubator.
  • You now can screen colonies for the plasmid.

  • 上一篇:PCR Amplification from Microbial Colonies   下一篇:Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌

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