For additional samples, move chamber select knob and repeat electroporation as above.
Turn off power buttons on both the power booster and pulse contol and disconnect cable on chamber safe. Remove suspended cells from chambers with a pipet and place in 1 mL rich media (SOC) to recover for 1 hr. at 37 °C in a shaker.
Plate 150 µL on appropriate plates (with antibiotic that corresponds to the resistance offered by your plasmid). Let grow overnight in 37 °C incubator.
You now can screen colonies for the plasmid.
上一篇:PCR Amplification from Microbial Colonies 下一篇:Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
共2页: 上一页 [1] 2 下一页