ml phenol/chloroform and vortex on 10 until t=2 minutes 10 seconds and move onto the next tube by t=2 minutes 20 seconds.ml Q and spin in the microfuge for 10 minutes. Remove 350-400 ml aqueous phase and add 0.1 volume 3M NaOAc 5.2, 1 ml tRNA and 2 volumes EtOH. ml formamide loading dye. Boil and load a 7% gel denaturing gel as for sequencing.
