12. Wash arrays as with mRNA labeling protocol and scan:
First wash: 2X SSC, 0.03% SDS, 5 min 65 °C
Second wash: 1X SSC, 5 min RT
Third wash: 0.2X SSC, 5 min RT
Note: the first washing step should be performed at 65° C; this appears to significantly increase the specific to non-specific hybridization signal.
If you have any questions, comments, or improvements, please pass them along to:
Jonathan R. Pollack, M.D., Ph.D.
Howard Hughes Medical Institute
Beckman Center B251
Stanford Medical Center
Stanford, CA 94305-5323
E-Mail jpollack@cmgm.stanford.edu
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