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The uncertainty created by this potential error can be dealt with through the following reasoning. The oligonucleotide probe is only one 21-base sequence. In each digest, it will hybridize only to the band that contains that sequence, so the bands that hybridize to the probe must contain an area of overlap. If students have to decide which of two bands actually hybridized to the probe, they can compare the bands’ locations in the lambda genome to the locations of the hybridizing bands from the other digests.Remember that the hybridizing bands in each of the digests must contain at
least one small common area. This area is the location of the probe sequence in the lambda genome.
Restriction site locations within the lambda chromosome(48,502 base pairs)
Further Reading
Bloom, M., G. Freyer, and D. Micklos. 1995. Laboratory DNA Science.
Benjamin/Cummings, Menlo Park, CA.
Kreuzer, H., and A. Massey. 2001. Recombinant DNA and Biotechnology:
A Guide For Teachers. American Society for Microbiology Press,
Washington, DC. (Carolina Biological Supply catalog #RN-21-2218)
Micklos, D., and G. Freyer, with David A. Crotty. 2003. DNA Science: A First
Course, 2nd Edition. Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, NY. (Carolina Biological Supply catalog #RN-21-2209)
Mullis, K. 1990. The unusual origin of the polymerase chain reaction.
Scientific American 262(4):56–65.
Nakamura, Y., M. Carlson, K. Krapco, and R. White. 1988. Isolation and
mapping of a polymorphic DNA sequence (pMCT118) on
chromosome 1p. Nucleic Acids Research 16:9364.
National Research Council. 1996. The Evaluation of
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