Integrating extrachromasomal arrays into the C. elegans chromosomes原理及方法[Da

8. When the F3s are old enough, look for a plate on which 100% of the F3s carry the array. This plate should carry a homozygous integrated array. I actually don't throw the plate out unless I see 2 worms that have lost the array; a single worm lacking the array might just be due to an unrelated L1 that was inadvertantly transferred to the plate along the with adult F2.

9. Since you put your worms through mutagenesis to generate this strain, you should backcross it several times to clean up the background.