Most of this protocol is derived from methods described in the following references:
Patel, N. H. and Goodman, C. S. (1992). Preparation of digoxigenin-labeled single-stranded DNA probes. In Non-radioactive Labeling and Detection of Biomolecules. (ed. C. Kessler). Springer-Verlag, Berlin.
Tautz, D. and Pfeifle, C. (1989). A nonradioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98, 81-85.
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