Laboratory of P.J. Hansen,Dept. of Animal Sciences, University of Florida
http://www.animal.ufl.edu/hansen/Protocols/live dead stain.htm
Procedures obtained from Ann Croy (Guelph, Canada) as a modification of procedures detailed in Transplantation 1971 12:148-151 Takasugi, M. An improved fluorochromatic cytotoxic test. Note that the procedure can be used for staining of all cells and not just embryos or oocytes. Also, ethididium bromide can be replaced with propidium iodide, DAPI or Hoescht 33342
Materials
Ethidium Bromide (EtBr; Fisher)
Fluorescein Diacetate (FDA; Sigma)
DPBS
Acetone
Procedure
1) Make following stock solutions:
EtBr (10 mg/mL DPBS) Store in dark at 4 C
FDA (5 mg/mL acetone) Store in dark in glass container at -20 C
Storage life of stocks ~4 months
2)
上一篇：FAM caspase 8 and 9 binding assay for embryos【University of Florida】 下一篇：Mini-Chamber for regulating gaseous environment during culture
共2页: 上一页 1 [2] 下一页
