Laboratory of P.J. Hansen,Dept. of Animal Sciences, University of Florida
http://www.animal.ufl.edu/hansen/IVF/default.htm
The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed in other laboratories at the University of Wisconsin (Parrish et al., 1986), University of Guelph (Xu et al., 1992), and University of Missouri (Hernandez-Ledezma et al., 1993). These procedures as used by our group have been published previously (Edwards et al., 1997; Paula-Lopes et al., 1998). Keep in mind that the protocols described here are not fixed but rather constantly evolve as new developments take place. Therefore, practitioners of IVP will be well advised to experiment with the procedures used, especially after reading of improvements made by other laboratories. This protocol is organized by day of the protocol with d 0 being the day of fertilization.
DAY-BY-DAY PROCEDURE
Day -2: Getting Prepared
Day -1: Collection of Ovaries | Oocyte Collection | Preparation of IVF Medium
Day 0: In Vitro Fertilization
Day 1-9: Culture of Embryos
Abbreviated Protocol for the Experienced User
Instructions for Printing This Manual
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