| Embedding, Sectioning, and Mounting of Frozen Tissues | | 点击： 作者：51protocol收集 来源： 时间： 2007-03-18 本站论坛 |
|  |
tissue-side of the slide is still visibly wet. Lay the slide flat on the paper towel,
and apply two drops of Crystal/Mount to the face of the slide opposite the labelside.
apply 2 drops here Immediately apply the 24x60 mm coverslip, left
edge first, so that the liquid/air line travels toward the right, leaving no bubbles
behind. Do not move the coverslip once it has been applied, because you may
disturb the sections and distort their morphology. Blot the edges of slide again
to absorb any excess liquid squeezed out during the application of the coverslip.
Let the slide sit for about 1 hour, or until the Crystal/Mount has hardened. Seal
the seam between the coverslip and the slide with nail polish to ensure that the
sections will not dry out.
上一篇：实验动物的给药途径和方法及药量计算方法 下一篇：Sample images of intestinal tract lesions, H 共2页: 上一页 [1] 2 下一页 | | |
|
